Development of a Commercial Laboratory Scale Soft X-ray Microscope
نویسندگان
چکیده
منابع مشابه
Laboratory soft-x-ray microscope for cryotomography of biological specimens.
Soft-x-ray cryotomography allows quantitative and high-resolution three-dimensional imaging of intact unstained cells. To date, the method relies on synchrotron-radiation sources, which limits accessibility for researchers. Here we present a laboratory water-window microscope for cryotomography. It is based on a λ=2.48 nm liquid-jet laser-plasma source, a normal-incidence multilayer condenser, ...
متن کاملLaboratory cryo soft X-ray microscopy.
Lens-based water-window X-ray microscopy allows two- and three-dimensional (2D and 3D) imaging of intact unstained cells in their near-native state with unprecedented contrast and resolution. Cryofixation is essential to avoid radiation damage to the sample. Present cryo X-ray microscopes rely on synchrotron radiation sources, thereby limiting the accessibility for a wider community of biologis...
متن کاملExperimental investigation of beam heating in a soft X-ray scanning transmission X-ray microscope.
A variable temperature sample holder with an operational range of 15 to 200 °C and an accuracy of ±1 °C has been fabricated for scanning transmission X-ray microscopes (STXM). Here we describe the device, and use it to image the polycrystalline morphology of solid stearic acid and palmitic acid at temperatures near their respective melting points as a means of checking for possible sample heati...
متن کاملOptimum driving a Z-pinch for soft X-Ray lasers
A capillary plasma z-pinch as an alternative active medium of soft X-Ray lasers was studied experimentally and theoretically. The theoretical analysis was based on the self consistent solution of the so called “snow plow” model. The dynamics of pinched plasma is determined by the capillary parameters and by the time dependence of electrical current passing through it. The current time dependenc...
متن کاملObservation of biological specimens using soft X-ray microscope
Biological specimens were observed using a soft X-ray microscope constructed at the Ritsumeikan synchrotron radiation center BL12. The spatial resolution of the microscope using an X-ray wavelength of 2.4nm was estimated to be 75nm from the edge response. Three kinds of diatoms were observed and ultrastructures of approximately 100nm could be clearly resolved. Two kinds of bacteria and red bloo...
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ژورنال
عنوان ژورنال: Microscopy and Microanalysis
سال: 2020
ISSN: 1431-9276,1435-8115
DOI: 10.1017/s143192762002351x